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High-performance liquid chromatographic assay of codeine in

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J Pharm Sci. 1980 Sep;69(9):1081-4. High-performance liquid chromatographic assay of codeine in acetaminophen with codeine dosage forms. Ko CY.

An accurate, rapid, and specific high-performance liquid chromatographic (HPLC) assay was developed for codeine in acetaminophen with codeine combination products. The internal standard (chlorpheniramine maleate), codeine, acetaminophen, and several other test compounds or impurities were well separated. A complete analysis took < 10 min. The relative standard deviations of the retention time, precision, and accuracy were 0.5, 0.4, and 0.5%, respectively. An excellent linear correlation was obtained between the HPLC and GLC methods.

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National Center for Biotechnology Information, U.S. National Library of Medicine 8600 Rockville Pike, Bethesda MD, 20894 USA.

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Determination of codeine in human plasma by high-performance

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A rapid, reliable and rugged assay for determining codeine in human plasma using reversed-phase high-performance liquid chromatography with fluorescence.

A rapid, reliable and rugged assay for determining codeine in human plasma using reversed-phase high-performance liquid chromatography with fluorescence detection was developed. This analytical method utilized an ion-exchange/mixed-mode solid-phase extraction procedure. The chromatographic separation was achieved using a 150 x 4.6 mm I.D., 3-microns reversed-phase C8 (deactivated for basic analytes) column at ambient temperature. Fluorescence detection (excitation at 214 nm and emission above 345 nm) for codeine and nalorphine allowed for a detectable limit of 5 ng/ml. The results showed that the method was linear from 10 to 300 ng/ml. The method had good reproducibility, precision, accuracy and recoveries of 91 and 90% for codeine and nalorphine, respectively. This method has been applied to study the pharmacokinetics of codeine in normal human subjects.

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National Center for Biotechnology Information, U.S. National Library of Medicine 8600 Rockville Pike, Bethesda MD, 20894 USA.

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11/17/2014
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